Premium
Blend membrane of regenerated silk fibroin, poly(vinyl alcohol), and peroxidase and its application to a ferrocene‐mediating hydrogen peroxide sensor
Author(s) -
Liu Yongcheng,
Qian Jianghong,
Liu Haiying,
Zhang Xiaolin,
Deng Jiaqi,
Yu Tongyin
Publication year - 1996
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19960725)61:4<641::aid-app7>3.0.co;2-l
Subject(s) - fibroin , vinyl alcohol , silk , membrane , hydrogen peroxide , peroxidase , polymer chemistry , ferrocene , chemical engineering , materials science , chemistry , polymer , electrochemistry , organic chemistry , composite material , electrode , enzyme , biochemistry , engineering
Before or after the blend membrane of regenerated silk fibroin (RSF), poly(vinyl alcohol) (PVA), and peroxidase is treated with ethanol, RSF, PVA, and peroxidase maintain their own structures. The conformational transition of RSF in the blend membrane is accomplished from the silk I structure to the silk II structure by ethanol treatment, which is used to immobilize peroxidase. A ferrocene‐mediating sensor for H 2 O 2 is made, which is based on the immobilization of peroxidase in the blend membrane of RSF and PVA. Performance and characteristics of the sensor were evaluated with respect to response time, detection limit, selectivity, and dependencies on temperature and pH as well as on operating and storage stability. © 1996 John Wiley & Sons, Inc.