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Characterization of poly[di(carboxylatophenoxy)‐phosphazene] by an aqueous gel permeation chromatography
Author(s) -
Andrianov Alexander K.,
Le Golvan Mark P.
Publication year - 1996
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/(sici)1097-4628(19960620)60:12<2289::aid-app28>3.0.co;2-0
Subject(s) - phosphazene , gel permeation chromatography , aqueous solution , chemistry , permeation , chromatography , characterization (materials science) , polymer chemistry , materials science , polymer , organic chemistry , membrane , biochemistry , nanotechnology
A water‐soluble phosphazene polyelectrolyte, poly[di(carboxylatophenoxy)phosphazene] (PCPP), was characterized using aqueous gel‐permeation chromatography (GPC) with concentration (UV and RI) and molecular weight sensitive (multiangle laser light‐scattering) detectors. Agreement was observed between the weight‐average molecular weights determined by GPC with a light‐scattering detector, conventional GPC using fractionated narrow PCPP standards, and also by static light‐scattering measurements. The effect of chromatography conditions, such as ionic strength of the mobile phase, column resolution, and injection volume was investigated. Mark–Houwink constants of PCPP in aqueous solution (phosphate buffer, pH 7.4, 0.42 M NaCl) were determined. The validity of the universal calibration curve and the occurrence of a secondary nonexclusion mechanism of separation in aqueous GPC of phosphazene polyelectrolytes are discussed. © 1996 John Wiley & Sons, Inc.