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Leukemia inhibitory factor, glial cell line‐derived neurotrophic factor, and their receptor expressions following muscle crush injury
Author(s) -
Kami Katsuya,
Morikawa Yoshihiro,
Kawai Yoshinori,
Senba Emiko
Publication year - 1999
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/(sici)1097-4598(199911)22:11<1576::aid-mus14>3.0.co;2-t
Subject(s) - glial cell line derived neurotrophic factor , leukemia inhibitory factor receptor , leukemia inhibitory factor , neurotrophic factors , axotomy , myocyte , endocrinology , in situ hybridization , crush injury , downregulation and upregulation , biology , medicine , neuroscience , receptor , central nervous system , gene expression , surgery , biochemistry , interleukin 6 , cytokine , gene
Using in situ hybridization histochemistry, we characterized the spatiotemporal gene expression patterns of leukemia inhibitory factor (LIF) and glial cell line‐derived neurotrophic factor (GDNF), and their receptor components (LIFR, GFR‐α1, RET) induced in muscle cells, intramuscular nerves, and motoneurons in the regeneration processes of both muscle cells and nerves following muscle contusion. Muscle contusion induced upregulation of GDNF and GFR‐α1 mRNAs in Schwann cell‐like cells in the intramuscular nerves and of LIFR mRNA in damaged muscle cells. LIFR, GFR‐α1, and RET mRNA expressions in motoneurons were upregulated following muscle contusion. Muscle contusion also induced more rapid, prominent transactivations of GFR‐α1 and RET genes in motoneurons than did sciatic nerve axotomy. These findings suggest that rapid and prominent upregulation of the receptor components for LIF and GDNF in motoneurons is important for the regeneration of intramuscular motor nerves damaged by muscle contusion. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 1576–1586, 1999