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Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle
Author(s) -
Sakai Yasutomo,
Iwamura Yukio,
Hayashi JunIchi,
Yamamoto Nao,
Ohkoshi Norio,
Nagata Hiroshi
Publication year - 1999
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/(sici)1097-4598(199902)22:2<258::aid-mus15>3.0.co;2-1
Subject(s) - mitochondrial dna , skeletal muscle , mitochondrion , mitochondrial myopathy , soleus muscle , biology , mitochondrial disease , polymerase chain reaction , exercise intolerance , medicine , anatomy , genetics , gene , heart failure
The present study was conducted to determine the effects of acute overload exercise on mitochondrial DNA and the structure of skeletal muscles. Rats were forced to run for 20 min until reaching complete exhaustion. We detected the large‐scale deletion (7052 bp) of mitochondrial DNA by the nested polymerase chain reaction, and also observed mitochondrial ultrastructural changes in the soleus muscle. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 258–261, 1999