Premium
Muscle precursor cells injected into irradiated mdx mouse muscle persist after serial injury
Author(s) -
Gross Jacqueline G.,
Morgan Jennifer E.
Publication year - 1999
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/(sici)1097-4598(199902)22:2<174::aid-mus5>3.0.co;2-s
Subject(s) - myogenesis , myocyte , regeneration (biology) , precursor cell , muscle tissue , chemistry , myotoxin , cell culture , cell , anatomy , microbiology and biotechnology , biology , biochemistry , genetics , phospholipase a2 , enzyme
Muscle of donor origin was formed after implantation of H‐2K b ‐tsA58 muscle precursor cells (mpc) into irradiated mdx nu/nu mouse muscles. A series of injections of the myotoxin, notexin, which destroys mature muscle fibers but spares muscle precursor cells and other tissues, was made into the mpc‐injected muscles, leaving time for regeneration to occur between each injection. New muscle fibers of donor origin were formed after up to four notexin treatments, providing evidence that some of the implanted mpc reentered an undifferentiated, quiescent, stem cell‐like state and were capable of myogenesis after further injuries to the muscle. A similar model could be used to assay whether preparations of human mpc contain long‐lasting precursor cells, prior to their implantation into patients. In control mdx muscles, which had been irradiated, injected with tissue culture medium, and given three notexin injections, regeneration also occurred, indicating that radiation‐resistant mpc were present, presumably within the treated muscle. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 174–185, 1999