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Increased interferon‐γmRNA expression following alloincompatible myoblast transplantation is inhibited by FK506
Author(s) -
Guérette Benoît,
Tremblay Geneviève,
Vilquin Jean Thomas,
Asselin Isabelle,
Gingras Marc,
Roy Raynald,
Tremblay Jacques P.
Publication year - 1996
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/(sici)1097-4598(199607)19:7<829::aid-mus3>3.0.co;2-b
Subject(s) - transplantation , major histocompatibility complex , interferon , immunology , biology , immune system , messenger rna , interferon gamma , antibody , histocompatibility , microbiology and biotechnology , antigen , medicine , human leukocyte antigen , gene , biochemistry
Myoblasts from C57BL/10SnJ+/+ were transplanted in major histocompatibility complex (MHC)‐compatible mice (i.e., C57BL/10SnJ+/+ and C57BL/10SnSc mdx/mdx) and in MHC‐noncompatible (BALB/c+/+) mice. The recipients were killed 1–21 days after transplantation. C57BL10SnJ+/+ myoblasts were also transplanted in a few BALB/c+/+ mice treated with FK506 and killed 7 days thereafter. Our results showed that after MHC‐noncompatible transplantation, interferon‐γ (IFN‐γ) mRNA expression is increased from day 5 to day 21, indicating the presence of a cellular immune reaction. Short‐term immunosuppressive treatment with FK506 inhibited the transcription of IFN‐γ mRNA compared with that in untreated mice. Myoblast‐specific antibodies were also detected 2 and 3 weeks after MHC‐incompatible transplantation, indicating that the cellular immune reaction, revealed by the increase in IFN‐γ, was followed by a humoral reaction. © 1996 John Wiley & Sons, Inc.