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Improving bioaffinity NMR spectra by means of zero‐quantum dephasing
Author(s) -
Weimar Thomas
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(200004)38:4<315::aid-mrc647>3.0.co;2-0
Subject(s) - dephasing , chemistry , coherence (philosophical gambling strategy) , zero (linguistics) , nmr spectra database , spectral line , quantum , nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , proton nmr , stereochemistry , quantum mechanics , physics , philosophy , linguistics
Bioaffinity NMR is based on the observation of transferred NOE (trNOE) effects which stem from bioactive components in a mixture containing a library of small compounds and a protein target. Zero‐quantum coherence signals can interfere with the trNOE cross peaks and, in the worst case, prevent the detection of a bioactive compound in a library. The dephasing of these zero‐quantum coherence signals during the experiment significantly improves the bioaffinity NMR spectra obtained and greatly reduces the risk of misinterpretations. Copyright © 2000 John Wiley & Sons, Ltd.