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31 P– 1 H correlation and resonance assignments along the DNA backbone: three‐dimensional implementation of heteronuclear long‐range correlation experiment
Author(s) -
Rastogi Vinit K.
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(200004)38:4<288::aid-mrc636>3.0.co;2-s
Subject(s) - heteronuclear molecule , spins , chemistry , nuclear magnetic resonance , pulse sequence , two dimensional nuclear magnetic resonance spectroscopy , resonance (particle physics) , range (aeronautics) , correlation , sequence (biology) , nuclear magnetic resonance spectroscopy , molecular physics , stereochemistry , physics , atomic physics , condensed matter physics , geometry , biochemistry , materials science , mathematics , composite material
A three‐dimensional heteronuclear NMR technique to correlate 31 P spins with 1 H along the backbone of DNA is described. The approach is based on a 31 P– 1 H heteronuclear long‐range correlation (HELCO) experiment in its refocused form. The utility of this technique is demonstrated by the sequence‐specific resonance assignment of 31 P and 1 H spins along the backbone of a mismatched base‐paired dodecanucleotide, d‐GGTACIAGTACC. Copyright © 2000 John Wiley & Sons, Ltd.