Premium
An improved J ‐multiplied method for the measurement of 3 J (H N , H α ) from the two‐dimensional 15 N– 1 H correlation spectrum
Author(s) -
Permi Perttu,
Kilpeläinen Ilkka,
Heikkinen Sami
Publication year - 1999
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199911)37:11<821::aid-mrc555>3.0.co;2-n
Subject(s) - chemistry , heteronuclear molecule , heteronuclear single quantum coherence spectroscopy , coherence (philosophical gambling strategy) , pulse sequence , quantum , relaxation (psychology) , coupling constant , statistical physics , nuclear magnetic resonance , quantum mechanics , analytical chemistry (journal) , molecular physics , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance spectroscopy , physics , stereochemistry , chromatography , psychology , social psychology
An improved method utilizing J ‐multiplication for the measurement of 3 J (H N , H α ) coupling constant is introduced. The current method benefits from slower relaxation rate of heteronuclear multiple‐quantum coherence. Furthermore, it combines incrementation periods for J ‐multiplication and 15 N chemical‐shift evolution. This reduces the length of the overall pulse sequence by t 1 . This, together with favourable relaxation properties of multiple‐quantum coherence, leads to narrower linewidths in the F 1 dimension relative to the previously presented sensitivity‐enhanced, J ‐multiplied HSQC experiment. The method is demonstrated on human ubiquitin. Copyright © 1999 John Wiley & Sons, Ltd.