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Gradient‐enhanced proton–fluorine NOE experiments
Author(s) -
Gerig J. T.
Publication year - 1999
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199909)37:9<647::aid-mrc520>3.0.co;2-n
Subject(s) - chemistry , nuclear overhauser effect , proton , fluorine , pulsed field gradient , coherence (philosophical gambling strategy) , fluorine 19 nmr , nuclear magnetic resonance , proton nmr , analytical chemistry (journal) , nuclear magnetic resonance spectroscopy , stereochemistry , molecule , organic chemistry , nuclear physics , physics , quantum mechanics
Uses of pulsed field gradients in the detection of 1 H{ 19 F} nuclear Overhauser effects in biological systems were examined in experiments wherein gradients were used for coherence selection and recall, as well as simply for the removal of unwanted magnetization components. Strategies for selective detection of these NOEs when the fluorine resonance is broad are proposed. Detection of 1 H{ 19 F} nuclear Overhauser effects in a carbonic anhydrase‐fluorinated inhibitor complex demonstrates the methods considered and shows the superiority of the “inverse” approach to detection of fluorine‐proton 1 H{ 19 } NOEs in macro‐molecular systems. Copyright © 1999 John Wiley & Sons, Ltd.