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Measurement of the deuterium relaxation times in double‐labeled ( 13 C/ 2 H) thymidine and 2′‐deoxyadenosine and in the selectively labeled DNA duplex 5′ d( 1 C 2 G 3 A 4 T 5 T 6 A 7 A 8 T 9 C 10 G) 2 3′
Author(s) -
Maltseva T. V.,
Földesi A.,
Chattopadhyaya J.
Publication year - 1999
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199903)37:3<203::aid-mrc433>3.0.co;2-v
Subject(s) - chemistry , deoxyadenosine , deuterium , thymidine , geminal , nucleoside , diastereomer , stereochemistry , dna , methylene , carbon 13 nmr , carbon 13 , proton , deuterium nmr , nucleotide , organic chemistry , biochemistry , physics , quantum mechanics , gene
The T 1 and T 1ρ of deuterium in 13 C/ 2 H double‐labeled 2′( R / S ),5′( R / S )‐ 2 H 2 ‐1′,2′,3′,4′,5′‐ 13 C 5 ‐2′‐deoxyadenosine and the corresponding thymidine derivative as well as in the non‐uniformly labeled (shown in bold and underlined) DNA duplex, d 5′ ( 1 C 2 G 3 A 4 T 5 T 6 A 7 A 8 T 9 C 10 G) 2 3′ , have been determined for the first time. These double‐labelled nucleoside blocks have a special feature in that the geminal 2′–2″ and 5′–5″ proton–proton couplings are eliminated by replacement with diastereomeric deuterium at C‐2′ and C‐5′ centers. This uniquely enables us to perform deuterium relaxation measurement experiments through selective polarization transfer, 1 H– 13 C– 2 H– 13 C– 1 H at C‐2′ and C‐5′ centers, thereby allowing filtration of all other naturally abundant methylene‐ and methine‐ 13 C as well as enriched methine‐ 13 C fragments. Comparison of T 1 and T 1ρ of 2 H in double‐labeled ( 13 C/ 2 H) 2′‐deoxyadenosine and thymidine with that of the non‐uniformly labeled DNA duplex, d 5′ ( 1 C 2 G 3 A 4 T 5 T 6 A 7 A 8 T 9 C 10 G) 2 3′ , shows that the dynamics of various nucleotide residues are indeed non‐uniform. Copyright © 1999 John Wiley & Sons, Ltd.