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Characterization of metabolites in plant extracts of Ancistrocladus likoko by high‐performance liquid chromatography coupled on‐line with 1 H NMR spectroscopy
Author(s) -
Bringmann Gerhard,
Rückert Markus,
Saeb Wael,
Mudogo Virima
Publication year - 1999
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199902)37:2<98::aid-mrc428>3.0.co;2-i
Subject(s) - chemistry , phytochemical , high performance liquid chromatography , nuclear magnetic resonance spectroscopy , chromatography , two dimensional nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , stereochemistry , biochemistry
One‐ and two‐dimensional stop‐flow HPLC–NMR experiments were applied to investigate a root extract of Ancistrocladus likoko (Ancistrocladaceae). Three new naphthylisoquinoline alkaloids with a 5,8′ coupling pattern were found. The constitutions and relative configurations were elucidated by application of H,H‐TOCSY and ROESY experiments carried out in the stop‐flow mode. This example demonstrates the potential of HPLC‐NMR as an efficient coupling technique for plant screening studies in phytochemical analysis. Copyright © 1999 John Wiley & Sons, Ltd.