Premium
Technical aspects of an efficient multiple solvent suppression pulse sequence
Author(s) -
Dalvit Claudio,
Shapiro Gideon,
Böhlen JeanMarc,
Parella Teodor
Publication year - 1999
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199901)37:1<7::aid-mrc398>3.0.co;2-f
Subject(s) - chemistry , solvent , pulse sequence , sequence (biology) , yohimbine , yield (engineering) , pulse (music) , spectral line , analytical chemistry (journal) , solvent effects , laminar flow , organic chemistry , nuclear magnetic resonance , thermodynamics , optics , receptor , astronomy , antagonist , biochemistry , physics , detector
The technical aspects of the excitation sculptingsequence with modification for multiple solvent suppression areanalyzed in detail. Proper choice of parameters for these experimentsyields spectra with almost complete absence of solvent signals. Thebleached regions centered at the solvent resonances are minimized byan appropriate selection of shape and length for the soft 180°shifted laminar pulses. Novel variants of the basic experiment withimproved performance for analysis of compounds dissolved inwater–alcohol mixtures are reported. Application of theseschemes to a 2 m M solution of α‐yohimbine in H 2 O–CH 3 CN and H 2 O–EtOH andto a few μ M solution derived from a natural productextract in H 2 O–CH 3 OH are presented.© 1999 John Wiley & Sons, Ltd.