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Conditions for the measurement of quantitative off‐resonance ROESY intensities
Author(s) -
Malliavin T. E.,
Desvaux H.,
Delsuc M.A.
Publication year - 1998
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(1998110)36:11<801::aid-omr367>3.0.co;2-j
Subject(s) - chemistry , spins , magnetization transfer , irradiation , resonance (particle physics) , magnetization , nuclear magnetic resonance , offset (computer science) , scalar (mathematics) , analytical chemistry (journal) , atomic physics , magnetic resonance imaging , condensed matter physics , magnetic field , physics , quantum mechanics , chromatography , medicine , geometry , mathematics , computer science , radiology , programming language
Two phenomena hamper the quantification of ROESYintensities: the off‐resonance effect and the coherent magnetizationtransfer between scalar‐coupled spins. The off‐resonance applicationof the r.f. irradiation has been proposed to reduce these artefacts.Here the reliability of this method for the quantification of ROESYintensities measured on a sample containing a macromolecule wasinvestigated. The threshold of irradiation offset for which thecoherent magnetization transfer can be neglected was determined. It isshown that for offsets larger than this threshold, the off‐resonanceeffect does not hinder the quantification, provided that theirradiation is alternatively applied at high and low field. ©1998 John Wiley & Sons, Ltd.