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Evaluation of the performance of 2D 1 H PFG absorption mode DQ spectroscopy in the analysis of chemical and biological molecules
Author(s) -
Dalvit Claudio,
Böhlen JeanMarc
Publication year - 1998
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199809)36:9<670::aid-omr356>3.0.co;2-s
Subject(s) - chemistry , sensitivity (control systems) , analytical chemistry (journal) , diagonal , absorption (acoustics) , spectroscopy , molecule , biological system , chromatography , optics , organic chemistry , physics , geometry , mathematics , quantum mechanics , electronic engineering , engineering , biology
The performance of the 1 H 2D PFG‐DQ experiment is analysed with theory and with some practical examples. The use of different angles for the detection pulse allows sensitivity optimization and spectral editing. Almost complete water suppression, high sensitivity, predominantly absorption lineshape, very low t 1 noise and absence of strong diagonal signals make the method very suitable for the analysis of complex mixtures, e.g. biofluids, cell cultures, natural product extracts and combinatorial chemistry samples. The method is also compared with the well known TOCSY and COSY experiments. © John Wiley & Sons Ltd.