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NMR spectroscopy of 13 C‐enriched polysaccharides: application of 13 C– 13 C TOCSY to sugars of different configuration
Author(s) -
Kjellberg Alexandra,
Nishida Toshiaki,
Weintraub Andrej,
Widmalm Göran
Publication year - 1998
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199802)36:2<128::aid-omr226>3.0.co;2-l
Subject(s) - chemistry , carbon 13 nmr , magnetization transfer , polysaccharide , proton , sugar , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , stereochemistry , organic chemistry , nuclear physics , physics , medicine , magnetic resonance imaging , radiology
13 C– 13 C 2D TOCSY experiments were applied, with both carbon‐13 and proton detection, to alleviate the problem of limited 1 H– 1 H coherence transfer in certain sugars, e.g. those with the manno or galacto configuration. The 13 C– 13 C couplings are not as dependent on the different sugar geometries and can be up to 50 times larger, i.e. 1 J CC vs . 3 J HH . For bacterial polysaccharides, M r ca . 10 4 –10 5 , loss of magnetization can be severe owing to long delays in 1 H– 1 H TOCSY experiments, and these are circumvented by applying the spin lock on the 13 C nuclei. © 1998 John Wiley & Sons, Ltd.