Premium
Optimization of LC–NMR. III—Increased signal‐to‐noise ratio through column trapping
Author(s) -
Griffiths Lee,
Horton Rob
Publication year - 1998
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199802)36:2<104::aid-omr230>3.0.co;2-f
Subject(s) - chemistry , solvent , column chromatography , deuterium , chromatography , analytical chemistry (journal) , column (typography) , proton nmr , signal (programming language) , phase (matter) , organic chemistry , physics , structural engineering , quantum mechanics , connection (principal bundle) , computer science , engineering , programming language
Significant gains in LC–NMR signal‐to‐noise ratio are obtained by selective concentration of chromatographic peaks on a chromatographic column using D 2 O to fix the sample on the solid phase. A deuterated organic solvent is used to remove the sample from the column and into the NMR flow cell, which removes the need for presaturation of solvent resonances. Deuterated solvents unrelated to the original eluent are used in order to minimize spectral overlap between solvent and solute. © 1998 John Wiley & Sons, Ltd.