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Direct qualitative and quantitative analysis of carbohydrate mixtures using 13 C NMR spectroscopy: application to honey
Author(s) -
Mazzoni Vanina,
Bradesi Pascale,
Tomi Félix,
Casanova Joseph
Publication year - 1997
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199712)35:13<s81::aid-omr202>3.0.co;2-e
Subject(s) - chemistry , fructose , nuclear magnetic resonance spectroscopy , qualitative analysis , chromatography , principal component analysis , carbon 13 nmr , carbohydrate , quantitative analysis (chemistry) , analytical chemistry (journal) , organic chemistry , qualitative research , social science , artificial intelligence , sociology , computer science
A method is described which allows the identification of individual carbohydrates of multi‐component artificial mixtures using the computer‐aided analysis of their 13 C NMR spectra, without previous separation. Quantitation of mono‐, di‐ and trisaccharides was carried out after improvement of the experimental procedure and using an internal standard. The procedure was then applied to authentic honeys of different floral types, harvested in Corsica (France). Several oligosaccharides (identification of some of these is not easy by chromatographic techniques) were observed at levels ranging from 0.4 to 3.3%. In addition, the fructose/glucose ratio, which has an influence on crystallization, was easily obtainable. © 1997 John Wiley & Sons, Ltd.