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Mobility of water in biological systems studied by 17 O NMR via multiple‐quantum filtered relaxation analysis
Author(s) -
Baguet Evelyne,
Hennebert Nicolas,
Chapman Bogdman E.,
Torres Allan M.,
Kuchel Philip W.
Publication year - 1997
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199712)35:13<s47::aid-omr188>3.0.co;2-e
Subject(s) - chemistry , bound water , relaxation (psychology) , macromolecule , lysozyme , aqueous solution , bovine serum albumin , proton nmr , nmr spectra database , water content , fraction (chemistry) , analytical chemistry (journal) , chemical physics , spectral line , thermodynamics , chromatography , organic chemistry , molecule , biochemistry , social psychology , physics , geotechnical engineering , astronomy , engineering , psychology
Multiple‐quantum filtering NMR sequences were used to study the multiexponential relaxation behaviour of H 2 17 O in the presence of macromolecules. By this means, the fraction and the correlation time of water in slow motion, or ‘bound’ water, were determined, in addition to the relaxation time of bulk water in the extreme narrowing limit. Experiments were carried out on aqueous solutions of bovine serum albumin, hen egg white lysozyme and intact human red blood cells in which intracellular water properties were studied. The small fraction of bound water of less than 1% seems to correspond to ‘strongly bound’ water, whereas the behaviour of bulk water was different from that of pure water and may be due to weak (or transient) interactions with macromolecules. The experiments and the data analysis gave reproducible results which support the idea that diverse samples could be studied in this way in order to help understand their water properties. © 1997 John Wiley & Sons, Ltd.