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Application of Semi‐Selective Excitation Sculpting for Homonuclear Decoupling During Evolution in Multi‐Dimensional NMR
Author(s) -
Krishnamurthy V. V.
Publication year - 1997
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199701)35:1<9::aid-omr930>3.0.co;2-r
Subject(s) - homonuclear molecule , chemistry , decoupling (probability) , excitation , pulse sequence , nuclear magnetic resonance , physics , quantum mechanics , organic chemistry , control engineering , molecule , engineering
A band‐selective homonuclear decoupled TOCSY sequence is described and compared with the standard TOCSY experiment, using a sample of a DNA 15mer. Band selection and homonuclear decoupling (BASHD) was achieved along the evolution dimension using a band‐selective double pulse field gradient spin echo (DPFGSE) technique. This semi‐selective excitation sculpting technique substantially improves the resolution and sensitivity of a crowded region in the TOCSY spectrum of the DNA 15mer chosen. The methodology described is general and can be implemented in other 2D and 3D techniques to achieve band selection and homonuclear decoupling during proton evolution. © 1997 by John Wiley & Sons, Ltd.