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Conformational Studies by 1 H NMR of the HIV Enhancer: the Transcription Factors NF‐κB and Sp1 Binding Domains
Author(s) -
Delepierre Muriel,
Sodano Patrick,
Gouyette Catherine,
Namane Abdelkader,
Igolen Jean,
Virelizier JeanLouis
Publication year - 1996
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199612)34:13<s67::aid-omr45>3.0.co;2-f
Subject(s) - chemistry , duplex (building) , dna , binding site , base pair , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , crystallography , transcription factor , stereochemistry , biophysics , gene , biochemistry , biology
The solution structures of two DNA non‐palindromic duplexes of 24 base pairs and corresponding to the 3′ NF‐κB‐binding site and the adjacent 5′ Sp1‐binding site of the HIV‐1 long terminal repeat (5′LTR) were analyzed by proton two‐dimensional nuclear magnetic resonance spectroscopy. The first sequence d(GGGGACTTTCCAGGGAGGCGTGGC):d(GCCACGCCTCCCTGGAAAGTCCCC) corresponds to the wild‐type LTR duplex (24mer‐N), and the second sequence d(GCTCACTTTCCAGGGAGGCGTGGC): d(GCCACGCCTCCCTGGAAAGTGAGC) (24mer‐M) contains a specific mutation (lightface letters) abolishing NF‐κB binding. Assignment of protons essential for structure determination were obtained and structural features of both duplexes were analyzed. The overall structure of the duplex is of the B form but several significant local structural deviations were found. First, from analysis of NOE cross‐peak intensities between adenine H2 base protons and sugar H1′ protons, it was found that the CTC mutation results in widening of the minor groove with presumably narrowing of the major groove, thus impairing the binding of NF‐κB to its responsive element in the HIV LTR. Second from chemical shift analysis of H1′ sugar protons, some unusual structural features were found at the junction between the homopurine:homopyrimidine stretches, that is, at the junction of the NF‐κB and Sp1 sites, consistent with the known synergism between NF‐κB and Sp1 functions in the HIV LTR. The scopes and limitations of DNA fragment studies of such a size are discussed.