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35 Cl NMR Study of Cl − Distribution and Transport in Human Red Blood Cell Suspensions
Author(s) -
Lin Wanrong,
de Freitas Duarte Mota
Publication year - 1996
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199610)34:10<768::aid-omr964>3.0.co;2-7
Subject(s) - chemistry , extracellular , intracellular , red blood cell , reagent , efflux , band 3 , kinetics , chloride , biophysics , chromatography , biochemistry , membrane , membrane protein , organic chemistry , physics , quantum mechanics , biology
The chloride (Cl − ) distribution in human red blood cell (RBC) suspensions was studied using cobalt(II) glycine, [Co(Gly) 3 ] − , as a shift reagent (SR). When [Co(Gly) 3 ] − was added to suspensions of human RBCs, it shifted the 35 Cl NMR signal of the extracellular Cl − . Intracellular and extracellular 35 Cl − signals were clearly discriminated. Erythrocytes were impermeable to [Co(Gly) 3 ] − but were slowly permeable to the SR Co 2+ and to the relaxation agent Mn 2+ . The exchange of intracellular Cl − with extracellular hypophosphite in human RBCs was monitored by 35 Cl NMR. The Cl − efflux was found to follow first‐order kinetics with an efflux rate constant of 0.0024 min −1 .