Premium
NMR Investigations of Fluorine‐Labelled RNA: Application of Two‐Dimensional Heteronuclear Experiments to Study Single‐Strand Conformation
Author(s) -
Kay Collier A.,
Arnold John R. P.,
Fisher Julie
Publication year - 1996
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199603)34:3<191::aid-omr856>3.0.co;2-i
Subject(s) - heteronuclear molecule , chemistry , two dimensional nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , stereochemistry , crystallography , physics
The novel application of 2D heteronuclear NMR experiments to a single‐stranded RNA fragment 5′‐GCAU*GGCU*ACU*U*‐3′ labelled with 5‐fluorouridine (U*) at four sites is reported. Heteronuclear equivalents of COSY and NOESY experiments are described, both implemented in the phase‐sensitive mode. The phase‐sensitive heteronuclear COSY experiment allows the observation of the 19 F– 1 H couplings, while comparison of heteronuclear NOESY spectra at various temperatures reveals exchange phenomena, not apparent in the magnitude mode or in one‐dimensional experiments. These methods and observations could have important implications for the study of conformational flexibility in larger, functionally relevant RNAs.