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Pulsed Field Gradient Pulse Sequence for Heteronuclear [ 31 P– 1 H] Long Range Correlation
Author(s) -
Keniry Max A.
Publication year - 1996
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/(sici)1097-458x(199601)34:1<33::aid-omr831>3.0.co;2-o
Subject(s) - heteronuclear molecule , pulse sequence , chemistry , nuclear magnetic resonance , pulse (music) , sequence (biology) , proton , range (aeronautics) , two dimensional nuclear magnetic resonance spectroscopy , spectral resolution , signal (programming language) , spectral line , analytical chemistry (journal) , molecular physics , nuclear magnetic resonance spectroscopy , physics , optics , stereochemistry , nuclear physics , materials science , biochemistry , chromatography , astronomy , detector , computer science , composite material , programming language
A proton‐detected heteronuclear 1 H– 31 P correlation experiment that uses z ‐pulsed field gradients to select coherences and suppress spectral artefacts is demonstrated for [d(G 3 T 4 G 3 )] 2 , an oligonucleotide which forms a 7 kDa DNA quadruplex in the presence of Na + and displays considerable spectral overlap. The pulse sequence, when applied to this quadruplex, displays a signal‐to‐noise ratio comparable to that with the most commonly used proton‐detected hereronuclear 1 H– 31 P correlation pulse sequence for macromolecules. Because the heteronuclear couplings are suppressed during the t 1 delay, linear prediction can be used in the F 1 dimension to improve the resolution of the spectrum.