Premium
Application of femtosecond coherence spectroscopy to the observation of nuclear motions in heme proteins and transparent solutions
Author(s) -
Wang Wei,
Demidov Andrey,
Ye Xiong,
Christian James F.,
Sjodin Theodore,
Champion Paul M.
Publication year - 2000
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(200001/02)31:1/2<99::aid-jrs500>3.0.co;2-b
Subject(s) - raman spectroscopy , myoglobin , wavenumber , coherent anti stokes raman spectroscopy , chemistry , coherence (philosophical gambling strategy) , spectroscopy , femtosecond , resonance raman spectroscopy , hemeprotein , heme , raman scattering , nuclear magnetic resonance , analytical chemistry (journal) , molecular physics , optics , laser , physics , organic chemistry , quantum mechanics , enzyme
The technique of femtosecond coherence spectroscopy (FCS) was applied to simple transparent organic fluids (chloroform and fenchone) and light‐absorbing heme proteins (myoglobin derivatives). This study demonstrates that time‐domain coherence measurements are in good agreement with standard frequency‐domain Raman and resonance Raman scattering experiments. The unique capability of FCS to detect the low‐wavenumber nuclear motions driven by the pumping fields and by the electronic state changes associated with photoinduced chemical reactions is also demonstrated. The latter response cannot be probed using traditional Raman spectroscopy. In all studies of myoglobin derivatives a low‐wavenumber mode was observed near 40 cm −1 . A sequence of low‐wavenumber modes was also observed near 80, 120 and 160 cm −1 that could be an indication of an overtone sequence. Copyright © 2000 John Wiley & Sons, Ltd.