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Surface‐enhanced Raman assays (SERA): measurement of bilirubin and salicylate
Author(s) -
Sulk Roberta,
Chan Collin,
Guicheteau Jason,
Gomez Cieline,
Heyns J. B. B.,
Corcoran Robert,
Carron Keith
Publication year - 1999
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199909)30:9<853::aid-jrs457>3.0.co;2-s
Subject(s) - raman spectroscopy , bilirubin , surface enhanced raman spectroscopy , chemistry , chromatography , medicine , optics , raman scattering , physics
Results of a study to detect bilirubin and salicylate using SERS and a reactive coating are presented. The motivation for this work was to devel to develop an assay that would simplify an analysis by detecting the analyte in a whole blood sample. This would remove the risks and time associated with preparing a serum sample. Quantitative analysis was performed using partial least‐squares. The results are that one can detect bilirubin and salicylate in whole blood at levels below the normal or therapeutic levels using a coating based on an argentiphilic diazonium molecule. The sharp Raman bands associated with the analyte–coating adduct are sufficiently resolved to allow the analyte to be detected in the presence of a complex matrix such as whole blood. Copyright © 1999 John Wiley & Sons, Ltd.