Premium
Raman difference studies of protein–nucleic acid interactions
Author(s) -
Deng Hua,
Callender Robert
Publication year - 1999
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199908)30:8<685::aid-jrs405>3.0.co;2-3
Subject(s) - raman spectroscopy , nucleic acid , chemistry , hydrogen bond , ab initio , nucleotide , computational chemistry , crystallography , molecule , organic chemistry , biochemistry , optics , physics , gene
Raman difference spectroscopy combined with isotope editing procedures were employed to obtain the Raman spectra of phosphates, nucleotides, nucleic acids and their fragments when bound to several proteins. The vibrational spectra were analyzed using a variety of empirical and ab initio methods to relate the vibrational wavenumbers to hydrogen bonding strengths, bond orders, bond lengths and geometry and to establish how the proteins bring about these interactions. Some recent results obtained using these methods are reviewed. Copyright © 1999 John Wiley & Sons, Ltd.