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Raman spectra of human skin and nail excited in the visible region
Author(s) -
de Faria D. L. A.,
de Souza M. A.
Publication year - 1999
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199903)30:3<169::aid-jrs365>3.0.co;2-p
Subject(s) - raman spectroscopy , stratum corneum , human skin , excited state , fluorescence , excitation , nail (fastener) , spectroscopy , characterization (materials science) , chemistry , infrared , materials science , analytical chemistry (journal) , optics , nanotechnology , atomic physics , physics , medicine , biology , metallurgy , genetics , pathology , quantum mechanics , chromatography
The characterization of human tissues for biomedical purposes usingspectroscopic techniques is of growing interest owing to the richnessof the information that they provide. Particularly in the case ofskin (stratum corneum) and nail, fluorescence has been adrawback to the use of Raman spectroscopy with excitation in thevisible and even in the red region, thus making the excitation in thenear‐infrared region and the use of interferometric equipmentessential. This paper reports for the first time the Raman spectra of theoutermost layer of human skin and nail excited at 632.8 nm. Theinitially strong fluorescent background was photobleached and theresidual emission was rejected by spatial filtering. The spectraobtained are in full agreement with previously reported data. Thefeasibility of excitation in the visible region increases thepossibilities of using Raman spectroscopy as a tool in skin and nailinvestigations. Copyright © 1999 John Wiley & Sons, Ltd.