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Resonance Raman labels and Raman labels
Author(s) -
Carey Paul R.
Publication year - 1998
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199810/11)29:10/11<861::aid-jrs323>3.0.co;2-b
Subject(s) - raman spectroscopy , resonance (particle physics) , resonance raman spectroscopy , chemistry , fluorescence , ligand (biochemistry) , raman scattering , analytical chemistry (journal) , biochemistry , optics , organic chemistry , physics , atomic physics , receptor
Resonance Raman labels are small chromophoric molecules that areplaced at biological sites as resonance Raman reporter groups. Thelabel may resemble closely a natural biological component, or it mayhave no biological counterpart and be placed at the site simply toprobe the properties of that site. Most of the early work withresonance Raman labels involved ligands binding to proteins, forexample chromophoric substrates or inhibitors binding to enzyme activesites. The sensitivity of Raman instrumentation has increased greatlyin recent years such that it no longer necessary to use the resonancecondition to obtain the Raman spectrum of a ligand binding to amacromolecule. These data can now be obtained from non‐resonancespectra by Raman difference spectroscopy. It is still advantageous ifthe ligand is a strong Raman scatterer and then the label becomessimply a Raman label. The advantages and disadvantages of resonanceand non‐resonance labels are discussed. For the latter, criticaladvantages of operating under non‐resonance conditions and using deepred excitation are that problems associated with photochemistry andfluorescence interference are avoided; consequently a wide range ofbiochemical systems become accessible to Raman analysis. Copyright © 1998 John Wiley & Sons, Ltd.