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NIR SERS detection of immune reaction on gold colloid particles without bound/free antigen separation
Author(s) -
Dou X.,
Yamaguchi Y.,
Yamamoto H.,
Doi S.,
Ozaki Yukihiro
Publication year - 1998
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199808)29:8<739::aid-jrs289>3.0.co;2-s
Subject(s) - colloidal gold , colloid , gold colloid , raman spectroscopy , chemistry , raman scattering , analytical chemistry (journal) , nanoparticle , materials science , nanotechnology , chromatography , optics , physics
This paper reports the near‐infrared (NIR) surface‐enhanced Raman scattering (SERS) detection of immune reaction on gold colloid particles without bound/free (B/F) antigen separation. A SERS spectrum was measured for anti‐mouse IgG at 2.2×10 ‐8 M adsorbed on gold colloid particles. The spectrum showed a number of Raman bands assignable to amide groups and aromatic amino acid residues of anti‐mouse IgG. A SERS spectrum of the same system at 1.9×10 ‐10 M did not yield any Raman band. However, Raman bands again appeared after the reaction of anti‐mouse IgG at 10 ‐10 M with the antigen on the gold colloid particles. The band intensity of the spectrum obtained was weak, but the spectral pattern was very similar to that of anti‐mouse IgG at 10 ‐8 M adsorbed on the gold colloid particles. Probably, the formation of the immune complex on the gold colloid particles changed the configuration of anti‐mouse IgG, enhancing the SERS signals. In this way, one could detect the immune complex on the gold colloid particles by NIR SERS spectroscopy without any B/F antigen separation. © 1998 John Wiley & Sons Ltd.