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Improved backscattering dual circular polarization raman optical activity spectrometer with enhanced performance for biomolecular applications
Author(s) -
Vargek Mária,
Freedman Teresa B.,
Nafie Laurence A.
Publication year - 1997
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199708)28:8<627::aid-jrs144>3.0.co;2-r
Subject(s) - raman spectroscopy , spectrograph , spectrometer , optics , raman optical activity , grating , detector , circular polarization , polarization (electrochemistry) , physics , chemistry , optoelectronics , spectral line , microstrip , astronomy
The results of an extensive upgrade of a backscattering dual circular polarization (DCP) Raman optical activity (ROA) spectrometer are reported. The instrument incorporates recent advances in electro‐optical technology for the measurement of Raman spectra. The primary improvements are the replacement of a f /4.2 Czerny–Turner spectrograph with an f /1.4 holographic transmission grating spectrograph, the replacement of a front‐illuminated CCD detector with a CCD system equipped with a larger, back‐thinned array and the substitution of a compensated quartz zeroth‐order quarter‐wave plate by an intrinsic zeroth‐order, polymer quarter‐wave plate. These improvements in throughput and detectivity have increased the speed of Raman spectral collection by approximately two orders of magnitude. In the most favorable case, one can see the basic features of the DCP I ROA spectrum of trans ‐pinane with a total Raman exposure time of only seconds. Examples of ROA spectra collected with the improved instrument are presented. © by John Wiley & Sons, Ltd.