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Raman Spectroscopic Study of Water in Lipid Dispersions: Changes in Structure of Hydrating Water Caused by Gel–Liquid Crystal Phase Transition
Author(s) -
Maeda Yasushi,
Kakinoki Kazuhiro,
Kitano Hiromi
Publication year - 1996
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/(sici)1097-4555(199605)27:5<425::aid-jrs975>3.0.co;2-p
Subject(s) - raman spectroscopy , hydrogen bond , chemistry , phase transition , dispersion (optics) , liposome , crystal structure , phase (matter) , crystal (programming language) , analytical chemistry (journal) , phospholipid , phosphatidylcholine , crystallography , molecule , chromatography , thermodynamics , organic chemistry , optics , biochemistry , physics , programming language , membrane , computer science
A Raman spectroscopic study was made of water structure in a L ‐α‐dimyristoylphosphatidylcholine (DMPC) liposome dispersion. By measuring the relative intensity I c of the peak corresponding to the collective O–H stretching mode of water in DMPC liposome dispersion at various temperatures, it was found that the degree of hydrogen bonding between water molecules decreased abruptly above 25°C, which corresponded to the liquid crystal–gel transition temperature of DMPC. In contrast, the degree of hydrogen bonding in solutions of L ‐α‐ dipropionyl‐phosphatidylcholine (DPrPC) and L ‐α‐glycerophosphorylcholine (PC), which do not exhibit phase transition phenomena in the temperature region examined, decreased linearly with increase in temperature. A critical change in water structure around the polar head of the DMPC liposome in the course of the phase transition is suggested.