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Expression pattern of galectin‐3 in neural tumor cell lines
Author(s) -
Kuklinski Stephan,
Pesheva Penka,
Heimann Cornelia,
Urschel Stephanie,
Gloor Sergio,
Graeber Sebastian,
Herzog Volker,
Pietsch Torsten,
Wiestler Otmar D.,
Probstmeier Rainer
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000401)60:1<45::aid-jnr5>3.0.co;2-y
Subject(s) - cell culture , biology , oligodendrocyte , glioma , galectin , oligodendroglioma , lectin , galectin 1 , galectin 3 , neural cell , microbiology and biotechnology , glial tumor , cell , cancer research , central nervous system , neuroscience , immunology , astrocytoma , myelin , genetics
Galectin‐3 is a member of the galectin family of β‐galactoside‐specific animal lectins. Here we show that galectin‐3 is constitutively expressed in 15 out of 16 glioma cell lines tested, but not by normal or reactive astrocytes, oligodendrocytes, glial O‐2A progenitor cells and the oligodendrocyte precursor cell line Oli‐neu. Galectin‐3 is also expressed by one oligodendroglioma cell line, but not by primitive neuroectodermal tumor and 4 neuroblastoma cell lines tested so far. In all galectin‐3 expressing cell lines, the lectin is predominantly, if not exclusively, localized intracellularly and carries an active carbohydrate recognition domain (shown for C6 rat glioma cells). Moreover, in contrast to primary astrocytes, glioma cells do not or only weakly adhere to substratum‐bound galectin‐3, probably reflecting an unusual glycosylation pattern. Our findings indicate that the expression of galectin‐3 selectively correlates with glial cell transformation in the central nervous system and could thus serve as a marker for glial tumor cell lines and glial tumors. J. Neurosci. Res. 60:45–57, 2000 © 2000 Wiley‐Liss, Inc.