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Differential distribution of the tetrodotoxin‐sensitive rPN4/NaCh6/Scn8a sodium channel in the nervous system
Author(s) -
Tzoumaka Elda,
Tischler Angela C.,
Sangameswaran Lakshmi,
Eglen Richard M.,
Hunter John C.,
Novakovic Sanja D.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000401)60:1<37::aid-jnr4>3.0.co;2-w
Subject(s) - sodium channel , sciatic nerve , spinal cord , tetrodotoxin , immunolabeling , chemistry , gene isoform , nervous system , dorsal root ganglion , neuroscience , sodium , nerve conduction velocity , anatomy , endocrinology , biophysics , medicine , biology , immunohistochemistry , biochemistry , organic chemistry , gene
Voltage‐gated sodium channels underlie the generation of action potentials in excitable cells. Various sodium channel isoforms have been cloned, functionally expressed and distinguished on the basis of their biophysical properties or differential sensitivity to tetrodotoxin (TTX). In the present study, we have investigated the immunolocalization of the TTX‐sensitive sodium channel, rPN4/NaCh6/Scn8a, in discrete areas of the rat nervous system. Thus, in naïve animals, PN4 was abundantly expressed in brain, spinal cord, dorsal root ganglia (DRG) and peripheral nerve. The presence of PN4 at the nodes of Ranvier in the sciatic nerve suggests the importance of this sodium channel in peripheral nerve conduction. In addition, the pattern of PN4 immunolabeling was determined in DRG, spinal cord and sciatic nerve in rats subjected to chronic constriction nerve injury (CCI). J. Neurosci. Res. 60:37–44, 2000 © 2000 Wiley‐Liss, Inc.