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Intracellular pH changes during oligodendrocyte differentiation in primary culture
Author(s) -
Boussouf Abdelhamid,
Gaillard Stéphane
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000315)59:6<731::aid-jnr5>3.0.co;2-g
Subject(s) - oligodendrocyte , intracellular ph , sodium–hydrogen antiporter , chemistry , cotransporter , intracellular , biophysics , microbiology and biotechnology , cellular differentiation , cell culture , biochemistry , sodium , biology , endocrinology , myelin , central nervous system , genetics , organic chemistry , gene
We have studied the characteristics of pH i regulation at different stages of rat oligodendrocyte differentiation in primary culture. pH i was measured at 37°C using the pH‐sensitive fluorescent probe BCECF. In immature oligodendrocyte progenitor (OLP), three distinct ionic mechanisms were involved in pH i regulation: (i) a sodium‐independent Cl − /HCO − 3exchanger, (ii) a Na + /H + exchanger and (iii) a voltage‐dependent Na + ‐HCO − 3cotransporter. The two latter mechanisms were also detected in more differentiated pro‐oligodendrocytes and in mature oligodendrocytes whereas the Cl − /HCO − 3exchanger was not active in these two later stages of differentiation. The presence of this Cl − /HCO − 3exchanger (that acts as a chronic acidifying mechanism) only in immature OLP maintains in these cells a steady‐state pH i value significantly lower than values measured in more differentiated cells. The possible involvement of this pH i change in triggering cell differentiation is discussed. J. Neurosci. Res. 59:731–739, 2000 © 2000 Wiley‐Liss, Inc.