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Lobster GABA receptor subunit expressed in neural tissues
Author(s) -
Hollins Bettye,
McClintock Timothy S.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000215)59:4<534::aid-jnr9>3.0.co;2-8
Subject(s) - picrotoxin , biology , gabaa receptor , complementary dna , ionotropic effect , bicuculline , protein subunit , cdna library , immunoscreening , gaba receptor , olfactory receptor , receptor , microbiology and biotechnology , biochemistry , glutamate receptor , gene
A cDNA encoding an ionotropic gamma‐aminobutyric acid (GABA) receptor subunit was isolated from a lobster ( Homarus americanus ) cDNA library. A longer version of this cDNA, containing a 108‐bp insert, was also detected. The two cDNAs are predicted to encode alternatively spliced proteins of 485 and 521 amino acids, respectively. The sequences were most similar to the Drosophila RDL (resistance to dieldrin) GABA subunit with 54% identity, and 30–35% identity with vertebrate ionotropic GABA receptor subunits. Only the shorter clone formed functional ion channels when transfected into human embryonic kidney (HEK) 293 cells. GABA caused a Cl ‐ ‐selective current in the presence of GABA that was blocked by picrotoxin. The GABA‐induced current was weakly sensitive to the GABA A antagonist, bicuculline, but was enhanced by pentobarbital. Expression of the GABA receptor mRNA was highest in brain and the olfactory organ, but was not detected in leg muscle. These data suggest that the isolated cDNAs are likely to encode proteins that comprise subunits of native GABA receptors expressed in olfactory receptor neurons and projection neurons of the olfactory deutocerebrum. J. Neurosci. Res. 59:534–541, 2000 © 2000 Wiley‐Liss, Inc.