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Differentiation of rat striatal embryonic stem cells in vitro: monolayer culture vs. three‐dimensional coculture with differentiated brain cells
Author(s) -
Pardo B.,
Honegger P.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000215)59:4<504::aid-jnr5>3.0.co;2-n
Subject(s) - embryonic stem cell , biology , microbiology and biotechnology , nestin , astrocyte , basic fibroblast growth factor , cellular differentiation , stem cell , cell culture , cell type , in vitro , lineage markers , neurosphere , neural stem cell , cell , adult stem cell , growth factor , neuroscience , progenitor cell , biochemistry , central nervous system , genetics , receptor , gene
Abstract Several groups have demonstrated the existence of self‐renewing stem cells in embryonic and adult mouse brain. In vitro, these cells proliferate in response to epidermal growth factor, forming clusters of nestin‐positive cells that may be dissociated and subcultured repetitively. Here we show that, in stem cell clusters derived from rat embryonic striatum, cell proliferation decreased with increasing number of passages and in response to elevated concentrations of potassium (30 mM KCl). In monolayer culture, the appearance of microtubule‐associated protein type‐5–immunoreactive (MAP‐5 + ) cells (presumptive neurons) in response to basic fibroblast growth factor (bFGF) was reduced at low cell density and with increasing number of passages. In the presence of bFGF, elevated potassium caused a more differentiated neuronal phenotype, characterized by an increased proportion of MAP‐5 + cells, extensive neuritic branching, and higher specific activity of glutamic acid decarboxylase. Dissociated stem cells were able to invade cultured brain cell aggregates containing different proportions of neurons and glial cells, whereas they required the presence of a considerable proportion of glial cells in the host cultures to become neurofilament H‐positive. The latter observation supports the view that astrocyte‐derived factors influence early differentiation of the neuronal cell lineage. J. Neurosci. Res. 59:504–512, 2000 © 2000 Wiley‐Liss, Inc.