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Analysis of oligodendroglial differentiation using cDNA arrays
Author(s) -
Scarlato Marina,
Beesley Jacqueline,
Pleasure David
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(20000201)59:3<430::aid-jnr18>3.0.co;2-7
Subject(s) - biology , microbiology and biotechnology , fyn , cellular differentiation , complementary dna , signal transduction , gene , biochemistry , tyrosine kinase
We used cDNA arrays to investigate molecular aspects of the differentiation of an immortalized line of oligodendroglial progenitors, and of immunopan‐purified primary cultures of oligodendroglial progenitors, to immature oligodendroglia. Developmental regulation of the proteolipid and 2‐hydroxyacylsphingosine 1‐galactosyltransferase genes was tighter in the primary than in the immortalized cells. Our data suggest that increased expression of genes encoding the following proteins are involved in oligodendroglial differentiation: Fyn, Erk, p85, G‐α‐12 guanine nucleotide binding, and transducin β‐2 signal transduction molecules; glial maturation factor; the proteasomal subunits C8 and C3; the proteasomal targeting molecule polyubiquitin; the cell cycle regulatory proteins Set, protein phosphatase 2A, and nuclear tyrosine phosphatase (PRL‐1); and the high‐affinity glutamate cotransporter EAAC‐1. J. Neurosci. Res. 59:430–435, 2000 © 2000 Wiley‐Liss, Inc.