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KIAA0369, doublecortin‐like kinase, is expressed during brain development
Author(s) -
Burgess Harold A.,
Martinez Salvador,
Reiner Orly
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19991115)58:4<567::aid-jnr9>3.0.co;2-t
Subject(s) - doublecortin , corticogenesis , biology , gene product , microbiology and biotechnology , gene , genetics , gene expression , dentate gyrus , hippocampus , neuroscience , embryonic stem cell
During embryonic development, the cerebral cortex attains its characteristic adult laminated structure. The finding that X‐linked lissencephaly patients harbor mutations in the doublecortin gene implicated this gene product in the process of corticogenesis. An autosomal human gene, KIAA0369, with a high level of similarity to doublecortin, has been cloned from human adult brain. This gene product contains a kinase domain in addition to a doublecortin‐like domain. In order to evaluate whether this doublecortin‐like kinase also plays a role during brain development, we cloned and studied the expression pattern of the mouse homolog. Three cDNA products of this gene were cloned: one, doublecortin‐like kinase, the second containing only the doublecortin‐like region, and the third containing only the kinase domain, a homolog of the previously cloned rat CPG16 gene. We studied doublecortin‐like kinase expression in mouse using Northern blot analysis, in situ hybridization, and Western blot analysis, and conclude that doublecortin‐like kinase is expressed in multiple regions of embryonic brain including the developing cerebral cortex. J. Neurosci. Res. 58:567–575, 1999. © 1999 Wiley‐Liss, Inc.