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Sampling of low molecular weight iron by microdialysis following spinal cord injury
Author(s) -
de Castro Romulo,
Alcock Nancy W.,
McAdoo David J.
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990901)57:5<735::aid-jnr15>3.0.co;2-#
Subject(s) - microdialysis , extracellular , spinal cord injury , spinal cord , chemistry , extracellular fluid , in vivo , biochemistry , biology , neuroscience , microbiology and biotechnology
We combined the use of desferrioxamine as an iron chelator, microdialysis sampling, and iron analysis by atomic absorption spectroscopy to measure extracellular levels of low molecular weight (LMW) iron in vivo in the spinal cord. Low molecular weight iron is free iron plus iron bound to small molecules. We show that the extracellular LMW‐iron concentration is not increased significantly in situ by trauma to the rat spinal cord, suggesting that an extracellular elevation in LMW‐iron at the site of injury is not the major initiating factor for lipid peroxidation following spinal cord injury. J. Neurosci. Res. 57:735–739, 1999. © 1999 Wiley‐Liss, Inc.