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Expression of colony stimulating factor‐1 receptor (CSF‐1R) by CNS neurons in mice
Author(s) -
Wang Y.Q.,
Berezovska O.,
Fedoroff S.
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990901)57:5<616::aid-jnr4>3.0.co;2-e
Subject(s) - spinal cord , central nervous system , neuron , downregulation and upregulation , in situ hybridization , brainstem , biology , receptor , neuroscience , cerebrospinal fluid , cerebral cortex , microglia , messenger rna , immunology , inflammation , biochemistry , gene
We report that neurons in the central nervous system express colony stimulating factor‐1 receptor (CSF‐1R) mRNA and protein and that the expression has regional specificity. The presence of CSF‐1R in neurons was demonstrated by the use of four different types of antibodies to CSF‐1R and the presence of CSF‐1R mRNA by in situ hybridization using oligonucleotide probe. In the steady state in most areas of the brain, CSF‐1R is weakly expressed in only a few neurons. In the cerebellum, brainstem, and spinal cord, however, CSF‐1R is expressed constitutively in greater numbers of neurons. After cerebral cortex ischemic injury, neurons in the area next to the ischemic lesion markedly upregulate CSF‐1R. It is also upregulated in the contralateral cortex and in many other areas of the brain and spinal cord. We demonstrated that in cultures the ligand CSF‐1 binds to its receptor (CSF‐1R) in neurons and that reduction of the number of apoptotic neurons and potentiation of neuron survival is CSF‐1 dose dependent. We propose that CSF‐1/CSF‐1R signaling is an important regulatory pathway between neurons, microglia, and astrocytes. J. Neurosci. Res. 57:616–632, 1999. © 1999 Wiley‐Liss, Inc.