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Expression and translocation of protein kinase C isoforms in rat microglial and astroglial cultures
Author(s) -
Slepko Natalia,
Patrizio Mario,
Levi Giulio
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990701)57:1<33::aid-jnr4>3.0.co;2-6
Subject(s) - protein kinase c , microglia , gene isoform , neuroglia , astrocyte , western blot , cytosol , microbiology and biotechnology , biology , cell type , isozyme , cell culture , cell , phorbol , kinase , biochemistry , immunology , central nervous system , endocrinology , enzyme , inflammation , gene , genetics
Cellular distribution and activation by phorbol myristate acetate (PMA) of classical (α, βI, βII,γ), novel (δ, ϵ, θ, η), and atypical (ζ, ι) protein kinase C (PKC) isoforms were studied in cultured rat neonatal microglial and astroglial cells by Western blot analysis. Among the classical isoforms, only βII was expressed in microglia and astrocytes in the same abundance. The expression of βI in microglia was less abundant, while PKCα was not detectable in this cell type. PKCγ was absent in both cell populations. A different pattern of expression was also found for novel and atypical isoenzymes: Both cell types expressed δ, θ, η, ζ, and ι isoforms, but PKCϵ was absent in microglia and the expression of PKCζ and PKCι in these cells was low compared to astrocytes. The pattern of PKC distribution in cytosolic and particulate fractions as well as activation by short (10 min) and prolonged (4 hr) PMA treatment in both cell types were similar. On the whole, in comparison with astrocytes, PKC in microglial cells was less expressed, both in terms of number of isoforms and level of expression. The microglial profile of PKC isoforms differed from that of rat peritoneal macrophages, which did express PKCα. Preliminary evidence suggests that the ability of PMA to enhance cyclic AMP responses in astrocytes, but not in microglia, is related to the different pattern of expression of PKCα and PKCϵ in the two cell types. J. Neurosci. Res. 57:33–38, 1999. © 1999 Wiley‐Liss, Inc.