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PC12 and neuro 2a cells have different susceptibilities to acetylcholinesterase–amyloid complexes, amyloid 25–35 fragment, glutamate, and hydrogen peroxide
Author(s) -
Calderón Frances H.,
Bonnefont Andrea,
Muñoz Francisco J.,
Fernández Virginia,
Videla Luis A.,
Inestrosa Nibaldo C.
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990615)56:6<620::aid-jnr8>3.0.co;2-f
Subject(s) - acetylcholinesterase , hydrogen peroxide , fragment (logic) , glutamate receptor , chemistry , aché , amyloid β , amyloid (mycology) , β amyloid , biochemistry , stereochemistry , biophysics , enzyme , alzheimer's disease , biology , medicine , computer science , receptor , inorganic chemistry , disease , programming language
This work addresses the differential effects of several oxidative insults on two neuronal cell lines, PC12 and Neuro 2a cells, extensively used as neuronal models in vitro. We measured cellular damage using the cytotoxic assays for MTT reduction and LDH release and found that acetylcholinesterase (AChE)–amyloid–β‐peptide (Aβ) complexes, Aβ 25–35 fragment, glutamate and H 2 O 2 were over 200‐fold more toxic to PC12 than to Neuro 2a cells. 17α and 17β estradiol were able to protect both cell types from damage caused by H 2 O 2 or glutamate. By contrast, other insults not related to oxidative stress, such as those caused by the nonionic detergent Triton X‐100 and serum deprivation, induced a similar level of damage in both PC12 and Neuro 2a cells. Considering that the Aβ peptide, H 2 O 2 and glutamate are cellular insults that cause an increase in reactive oxygen species (ROS), the intracellular levels of the antioxidant compound, glutathione were verified. Neuro 2a cells were found to have 4‐ to 5‐fold more glutathione than PC12 cells. Our results suggest that Neuro 2a cells are less susceptible to exposure to AChE–Aβ complexes, Aβ 25–35 fragment, glutamate and H 2 O 2 than PC12 cells, due to higher intracellular levels of antioxidant defense factors. J. Neurosci. Res. 56:620–631, 1999. © 1999 Wiley‐Liss, Inc.