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Differential mRNA expression and subcellular locations of PI3‐kinase isoforms in sympathetic and sensory neurons
Author(s) -
Bartlett Selena E.,
Reynolds Anna J.,
Tan Timothy,
Heydon Katharina,
Hendry Ian A.
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990401)56:1<44::aid-jnr6>3.0.co;2-6
Subject(s) - wortmannin , gene isoform , growth cone , nerve growth factor , microbiology and biotechnology , superior cervical ganglion , biology , sensory neuron , dorsal root ganglion , kinase , phosphatidylinositol , neuroscience , sensory system , endocrinology , axon , receptor , gene , biochemistry
Phosphatidylinositol 3‐kinase (PI3‐kinase) enzymes are key signalling molecules in the PC12 and neuronal cell survival pathway and are also involved in the regulation of retrograde axonal transport of nerve growth factor (NGF), with sympathetic neurons more sensitive to the effects of wortmannin/LY294002 than sensory neurons (Bartlett et al. [1997]; Brain Res. 761:257–262; Reynolds et al. [1998] Brain Res. 798:67–74). In this article, we characterized the mRNA expression of PI3‐kinase isoforms in mouse sympathetic superior cervical ganglia (SCG) and sensory trigeminal ganglia (TGG) and examined the subcellular locations of immunoreactivity of the PI3‐kinase isoforms in mouse cultured SCG and dorsal root ganglion (DRG) neurons. Both the SCG and the TGG express mRNA for the p110α, β, γ, δ, and vps34p PI3‐kinase isoforms, but the TGG and not the SCG express mRNA for the p170 PI3‐kinase isoform. In cultured SCG and DRG neurons, p110α, β, and γ immunoreactivity is in the SCG and DRG growth cones, and predominantly in puncta throughout the growth cone varicosity. However, in the cell bodies immunoreactivity varied, p110α is localized predominantly at the plasma membrane, while p110β and γ is localized in the perinuclear region of the cells. In addition, unlike other cell types, wortmannin has little effect on actin filament polymerization in either mouse cultured SCG or DRG neurons. J. Neurosci. Res. 56:44–53, 1999. © 1999 Wiley‐Liss, Inc.