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Cardiotrophin‐1 requires LIFRβ to promote survival of mouse motoneurons purified by a novel technique
Author(s) -
Arce Vilma,
Garces Alain,
de Bovis Béatrice,
Filippi Pierre,
Henderson Christopher,
Pettmann Brigitte,
deLapeyrière Odile
Publication year - 1999
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19990101)55:1<119::aid-jnr13>3.0.co;2-6
Subject(s) - ciliary neurotrophic factor , glycoprotein 130 , microbiology and biotechnology , leukemia inhibitory factor , receptor , biology , leukemia inhibitory factor receptor , transmembrane protein , signal transduction , cell sorting , neurotrophin , neuroscience , neurotrophic factors , cell , cytokine , immunology , biochemistry , stat3 , interleukin 6
The cytokines ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) signal through a receptor complex formed between two transmembrane proteins, gp130 and LIFRβ. In addition, CNTF also uses a ligand‐binding component which is anchored to the cell membrane. In the case of cardiotrophin‐1 (CT‐1), LIFRβ is also required in cardiomyocytes, but this has not been proven in neurons, and published data suggest that motoneurons may use a different receptor complex. We used Lifr β knockout mice to assess the requirement for this receptor component in the signal transduction of CT‐1 in motoneurons. To study purified motoneurons from such mutants, we have developed a method allowing for isolation of highly purified mouse motoneurons. This protocol is based on the immunoaffinity purification of motoneurons using antibodies against the extracellular domain of the neurotrophin receptor, p75, followed by cell sorting using magnetic microbeads. We show that CNTF, LIF, and CT‐1 are unable to promote the survival of motoneurons derived from homozygous Lifr β −/− mutant embryos. Thus, LIFRβ is absolutely required to transduce the CT‐1 survival signal in motoneurons. J Neurosci Res 55:119–126, 1999. © 1999 Wiley‐Liss, Inc.