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Docosahexaenoic acid decreases phospholipase A 2 activity in the neurites/nerve growth cones of PC12 cells
Author(s) -
Martin Rex E.
Publication year - 1998
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19981215)54:6<805::aid-jnr8>3.0.co;2-4
Subject(s) - neurite , docosahexaenoic acid , phospholipase a2 , chemistry , nerve growth factor , microbiology and biotechnology , growth cone , neuroscience , phospholipase , nerve cells , biophysics , biochemistry , biology , enzyme , axon , fatty acid , polyunsaturated fatty acid , receptor , in vitro
Docosahexaenoic acid (DHA) accumulates in nerve growth cones (NGC) during perinatal development and it is neuroprotective in ischemia. Because the phospholipases A 2 (PLA 2 ) are present in NGC and these enzymes function in both ischemia and long‐term potentiation, the relationship between DHA and PLA 2 was investigated in the NGC of nerve growth factor‐differentiated PC12 cells. When PC12 cells were incubated with [ 3 H]DHA, it primarily esterified in ethanolamine glycerolipids and concentrated initially in cell bodies with similar levels present in the neurite/nerve growth cone (N/NGC) fraction after 4 days. PLA 2 activity in the N/NGC fraction was investigated using [ 14 C]arachidonic acid‐labeled phosphatidylinositol ([ 14 C‐AA]PI) as substrate. Heat denaturation and pharmacological inhibition showed that much of the PLA 2 activity was calcium‐independent and secretory rather than cytosolic. Supplementing the media with as little as 33 nM DHA significantly reduced PLA 2 activity in the N/NGC fraction. J. Neurosci. Res. 54:805–813, 1998. © 1998 Wiley‐Liss, Inc.