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Differential turnover of syntaxin and SNAP‐25 during synaptogenesis in cultured cerebellar granule neurons
Author(s) -
Sanders Jeff D.,
Yang Yuanzheng,
Liu Yuechueng
Publication year - 1998
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19980915)53:6<670::aid-jnr5>3.0.co;2-2
Subject(s) - synaptogenesis , syntaxin , microbiology and biotechnology , biology , snap , neuroscience , granule (geology) , vesicle , biochemistry , membrane , computer science , computer graphics (images) , paleontology
In order to investigate the molecular mechanism underlying synaptogenesis, we examined the dynamics and stability of syntaxin 1A and SNAP‐25 in cultured cerebellar granule cells. In neurons cultured for less than 5 days in vitro (DIV), syntaxin was highly expressed with a half‐life of >48 hours. SNAP‐25 was also expressed at 5 DIV, but at a lower level and with a much shorter half‐life of 16 hours. As the neurons matured and established synpatic connections, the expression of both proteins increased steadily, with the more rapid increase between 5 DIV and 8 DIV associated with SNAP‐25. The half‐life of syntaxin was slightly increased in the mature neurons. SNAP‐25, however, showed an increased half‐life of about 35 hours. These results suggested that the dynamics and stability of the t‐SNAREs are differentially modulated during synaptogenesis, which may be important in establishing and maintaining synaptic connections. J. Neurosci. Res. 53:670–676, 1998. © 1998 Wiley‐Liss, Inc.