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Seizure activity induces PIM‐1 expression in brain
Author(s) -
Feldman J.D.,
Vician L.,
Crispino M.,
Tocco G.,
Baudry M.,
Herschman Harvey R.
Publication year - 1998
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19980815)53:4<502::aid-jnr13>3.0.co;2-3
Subject(s) - kainic acid , forskolin , dentate gyrus , depolarization , stimulation , medicine , endocrinology , biology , hippocampus , messenger rna , chemistry , microbiology and biotechnology , neuroscience , gene , receptor , biochemistry , glutamate receptor
We recently identified KID‐1, a previously undescribed protein kinase induced by depolarization in PC12 cells and brain (Feldman et al., 1998). KID‐1 shares a high degree of sequence homology with PIM‐1, a proto‐oncogene previously reported to be expressed in hematopoietic and germ cells. We examined PIM‐1 expression in stimulated PC12 cells, brains of kainic acid‐treated rats, and a number of tissues from untreated rats. We now report that forskolin, but not depolarization or growth factors, induces PIM‐1 expression in PC12 cells. PIM‐1 is an immediate early gene induced in response to forskolin stimulation. We detect PIM‐1 mRNA in a number of unstimulated tissues and at low levels in unstimulated brain. Systemic kainic acid administration to adult rats induces PIM‐1 expression in the dentate gyrus region of the hippocampus. J. Neurosci. Res. 53:502–509, 1998. © 1998 Wiley‐Liss, Inc.