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Proximal promoter region is sufficient to regulate tissue‐specific expression of UDP‐galactose: Ceramide galactosyltransferase gene
Author(s) -
Yonemasu Tomoko,
Nakahira Kensuke,
Okumura Syunichiro,
Kagawa Tetsushi,
Espinosa de los Monteros Araceli,
de Vellis Jean,
Ikenaka Kazuhiro
Publication year - 1998
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19980615)52:6<757::aid-jnr15>3.0.co;2-w
Subject(s) - galactosyltransferase , microbiology and biotechnology , gene , gene expression , regulation of gene expression , biology , galactose , genetics , biochemistry , enzyme
UDP‐galactose:ceramide galactosyltransferase (CGT) is the enzyme which catalyzes the final step of the biosynthesis of galactocerebroside (GalC), the most abundant glycolipid in myelin. We identified regulatory elements which are related to the tissue‐specific expression of the mouse CGT gene by promoter assay using chimeric CGT‐luciferase constructs. By comparing promoter activity in oligodendroglial CG4 cells and NIH3T3 fibroblasts, only a few hundred base pairs spanning from −309 to −98 were shown to be necessary for the tissue‐specific activity of CGT promoter. A negative regulatory element was found in a more distal region, from −709 to −527, and it also worked in tissue‐specific manner. Sequence analysis suggests that several known elements found commonly in myelin‐related genes may explain these tissue‐specific regulations of the transcriptional activity. J. Neurosci. Res. 52:757–765, 1998. © 1998 Wiley‐Liss, Inc.