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Differentiation of oligodendrocyte occurs in contact with astrocyte
Author(s) -
Sakurai Yoko,
Nishimura Daisuke,
Yoshimura Kazuhiro,
Tsuruo Yoshihiro,
Seiwa Chika,
Asou Hiroaki
Publication year - 1998
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/(sici)1097-4547(19980401)52:1<17::aid-jnr3>3.0.co;2-n
Subject(s) - oligodendrocyte , astrocyte , microbiology and biotechnology , biology , in vitro , cellular differentiation , cell culture , immunology , neuroscience , myelin , central nervous system , biochemistry , gene , genetics
We have generated stage‐specific oligodendrocytes, which are identified as immature oligodendrocytes by the expression of specific markers. These cells were generated by using 3 types of intrinsic and extrinsic factors for influencing the characteristics of oligodendrocyte‐lineage cells. The factors included 10% serum in the medium to induce cell proliferation, bFGF in serum‐free medium to increase survival and induce differentiation, and 0.25% trypsin to remove nonoligodendrocyte lineage cells. We have also demonstrated that it is possible to supply a huge amount of homogeneous populations of stage‐specific immature oligodendrocytes after 35 days' culture. The maturation of these cells is controlled by interacting with astrocytes in vitro, resulting in the expression of MBP isoforms (14.0 and 18.5 kDa) that may participate in mature oligodendrocyte membrane compaction. J. Neurosci. Res. 52:17–26, 1998. © 1998 Wiley‐Liss, Inc.